Journal: Journal of microbiological methods
Article Title: SILKWORM LARVAE PLASMA (SLP) ASSAY FOR DETECTION OF BACTERIA: FALSE POSITIVES SECONDARY TO INFLAMMATION IN VIVO
doi: 10.1016/j.mimet.2016.11.009
Figure Lengend Snippet: (A) Intranasal inoculation of wild-type mice with live (black bars) or heat-inactivated (grey-bars) of L. plantarum followed by evaluation of lung tissue at time points as in Fig. 1; no L. plantarum control is shown in blue at t = 0; n = 3 mice per group. SLP assay as described in Methods, interpolated to a standard curve as in Fig. 2 is generated for each experiment. (B) Intranasal inoculation of wild-type (blue bars) or NOD2-TLR2 gene-deleted mice (NOD2−/−TLR2−/−; grey bars) with heat-inactivated L. plantarum followed by evaluation of lung tissue at time points indicate; n = 3 mice per group. (C) Inoculation of wild-type mice with soluble bi-functional pattern-recognition receptor agonist CL-429 (20 µg per mouse in 50 µL pbs/bsa) followed by evaluation of mouse lung tissue at time points indicated; n = 3 - 4 mice per group, *p<0.01, **p < 0.001 by ANOVA.
Article Snippet: The bifunctional NOD2-TLR2 agonist, CL-429 (Invivogen) was diluted in PBS/BSA and delivered to isoflurane-anaesthetized mice (20 µg/ 50 µL per mouse) in parallel with appropriate diluent controls.
Techniques: Generated, Functional Assay